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Hypoxyprobe™ Plus Kit說明書

發(fā)布時間:2018/5/7      點(diǎn)擊次數(shù):1943

實(shí)驗(yàn)材料供應(yīng)商 Hypoxyprobe正式授權(quán)上海起發(fā)為其中國代理, Hypoxyprobe在一直是行業(yè)的*,一直為廣大科研客戶提供zui為的產(chǎn)品和服務(wù),上海起發(fā)一直秉承為中國科研客戶帶來好的產(chǎn)品, 好的服務(wù),簽約 Hypoxyprobe就是為了給廣大科研客戶帶來更加完善的產(chǎn)品和服務(wù),您的滿意將是我們zui大的收獲

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Hypoxyprobe™ Plus Kit

(HPI Part # HP2-XXX)

Kit contents:

Solid pimonidazole HCl (Hypoxyprobe™-1)

FITC conjugated to anti-pimonidazole mouse IgG1 monoclonal antibody (FITC-MAb1) and

Rabbit anti-FITC conjugated with horseradish peroxidase as a secondary reagent.

Applications: Immunochemical detection of cell and tissue hypoxia through immunofluorescence or immunoperoxidase staining, western blotting, or flow cytometry. There is very low background in mouse tissues when the Hypoxyprobe™ Plus Kit is combined with an anti-FITC secondary reagent.

Quantities: a. Hypoxyprobe™ Plus Kits contain100 mg, 200 mg or 1000 mg of pimonidazole HCl. Typical doses are 60mg/kg body weight in small animal studies and 14mg/kg body weight for human studies.  b. One vial (100 and 200 mg Kits) or two vials (1000 mg Kit) containing 200 uL of an anti-pimonidazole, FITC-conjugated IgG1 mouse monoclonal antibody (clone 4.3.11.3). Each vial contains 200 microliters of a 0.50 mg/ml solution of the FITC-MAb1 in PBS containing 1 % BSA and 0.09% sodium azide. Optimal dilution of the FITC-MAb1 is to be determined by the investigator but 1:50 or 1:100 dilutions have been found to give strong immunostaining in mouse tumor tissue when combined with a 1:50 or 1:100 dilution of the peroxidase conjugated anti-FITC secondary reagent. While not designed specifically for immunofluorescence, FITC-MAb1 can be used for this purpose.  Antibody dilution is typically lower for immunofluorescence than for immunoperoxidase detection.

 

Not supplied: Standard reagents used for immunohistochemical analyses.

 

Storage: a. Store Hypoxyprobe™-1 solid at room temperature in the dark (can also be stored at 2- 8 degrees C).  b. Store FITC-MAb1 and HRP conjugated rabbit anti-FITC at 2-8 degrees C in the dark.    Do not freeze. 

 

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1:基本什么都能進(jìn)口,血清,抗體,耗材,還有部分限制進(jìn)口的,

2:貨品全,現(xiàn)經(jīng)營過700多個品牌,基本所有生物試劑耗材都可以進(jìn)口,特別是冷偏的產(chǎn)品那就更有優(yōu)勢,

3:提供加急服務(wù),一般1-2周到貨,超過時限加急費(fèi)全免

4:價(jià)格公道,絕大部分價(jià)格有優(yōu)勢,當(dāng)然不能保證100%產(chǎn)品都是,因?yàn)橐馕吨鴽]有服務(wù).

5:良好的信譽(yù),大部分客戶我們提供貨到付款服務(wù),客戶包括清華,北大 交大 復(fù)旦,中山等100多所大學(xué),ROCHE,阿斯利康,國藥 ,fisher等500多家公司

6:我們*代理的品牌有:Antibody Research Corporation,arcticzymes,Biorelevant,AmberGen, Inc. ,clemente-associates,clodronaiposomes,Columbia Biosciences,enzyme research,Gene Bridges GmbH,Genovis,AmberGen, Inc.  Biotechnology GmbH,Haematologic Technologies HTI Haemtech,hookelabs,Immudex,Innovative Research of America,inspiralis ,List Biological Laboratories, Inc.,lumafluor,Microsurfaces,multiplicom,nanotools,Pel-Freez Biologicals,pentapharm,progen,Protein Ark,Hypoxyprobe,Inc,Hypoxyprobe,IncQuickZyme Biosciences,Teknova,TriLink BioTechnologies, Inc.,Zyagen Laboratories 等

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